gan choon kiat lta

Teh, S.T. However, this phenomenon was not observed in the K562 cells in which STAT5 phosphorylation is dependent on BCR/ABL1 (8) and not JAK3 (Fig. View Ang Choon Kiat Amos’ profile on LinkedIn, the world’s largest professional community. Furthermore, Annexin V staining revealed that the reduction of NK-S1 viability was due to an increase in cellular apoptosis (Fig. Sequencing PCR was carried out with ABI BigDye Terminator v3.1 (Life Technologies, catalog number 4337457) and cycled at 96°C for 1 minute; 29 cycles of 96°C for 10 seconds; 50°C for 5 seconds, and 60°C for 4 minutes. ): G.C. The JAK1Y652D mutation was located in the JH2 domain as well. Treatment with CP-690550 resulted in reduced cell viability of NK-S1 and KHYG-1 cells but not in K562 cells. A research-intensive university with an entrepreneurial dimension, NUS is ranked consistently as one of the world's top universities. Sanger sequencing and HRM (25, 26) were used to confirm the JAK3 and JAK1 mutations identified and validate their prevalence in our NKTCL patient population. Ng, V. Rajasegaran, H.L. NK-S1, established from a previously described NKTCL xenograft (27), was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Life Technologies) supplemented with 10% heat-inactivated FBS and 10% equine serum. Facebook gives people the power to share and makes the world more open and connected. There has been little progress in basic science and clinical research in this subtype of lymphoma, which continues to constitute a major challenge in managing these patients as there is currently no accepted standard first-line treatment for NKTCLs. All 3 missense mutations were predicted by PolyPhen to be damaging (5). Consistent with the high frequency of JAK3 mutations (35%) in NKTCLs, use of CP-690550 in the JAK3-mutant NKTCL cell line showed inhibition in the phosphorylation of STAT5 along with reduced cell viability. *, P < 0.05 compared with vehicle control (vehicle). Further validation of the prevalence of JAK3 mutations was determined by Sanger sequencing and high-resolution melt (HRM) analysis in an additional 61 cases. and was admitted to the Singapore Bar in 1978. Gan Choon Kiat, deputy director of the LTA's corporate transformation office, was quoted by Channel NewsAsia as saying that more themed trains will be rolled out in future. After blocking, membranes were probed with primary antibodies against phospho-STAT5 (Cell Signaling, catalog number 9356), STAT5 (Cell Signaling, catalog number 9363), phospho-JAK3 (Cell Signaling, catalog number 5031), JAK3 (Cell Signaling, catalog number 3775), and β-actin followed by either peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody. B, KHYG-1 cells were transiently transfected with wild-type JAK3 (JAK3 WT) or mutated JAK3 expression vectors (i.e., JAK3A572V). In contrast, JAK3 wild-type KHYG-1 cells were clearly IL-2–dependent (Fig. Gan Ja. Find contact's direct phone number, email address, work history, and more. The JAK/STAT pathway is a key component in normal hematopoiesis. These results indicate that the JAK3-activating mutations are gain-of-function alleles and contribute to the constitutive activity of the JAK/STAT pathway in an IL-2–independent manner. Tan, S.L. This is the first time that the Land Transport Authority (LTA) has introduced a festive-themed train. Commuters can also grab the Christmas tree-shaped cards hanging from the handrails to use as greeting cards. JAK-mutant (NK-S1) cells showed IL-2–independent growth (Fig. Heng, A. Gan, S.T. These are some of the notable cases which SPD officers handled in 2011: In PP v Abdul Razak Bin Hamid and PP v Tan Chye Guan Martin, the accused persons were both heinous child molesters, dubbed the “Letterbox Molester” and “Heartbeat Molester” respectively.They engaged in ruses to con children into physical contact and committed the offences over 20 years and 11 years respectively. Among these 4 members, JAK3 signaling is specifically related to T-cell development and proliferation (8) with loss-of-function mutations resulting in severe combined immunodeficiency characterized by the lack of T and NK cells (9). Total proteins were extracted with lysis buffer, resolved by SDS-PAGE gels, and blotted onto a nitrocellulose membrane. Join Facebook to connect with Choon Kiat Gan and others you may know. Mr Gan first joined ComfortDelGro Corporation Limited as Group Corporate Planning Officer in February 2006. Yap, K.A. Poon, G.E. This study is dedicated to Dr. Han Mo Koo. 2A) and constitutive phosphorylation of both JAK3 and STAT5 (Fig. "Every month or so we will be working with our public transport operators to have more themed trains in the next year," he said. CP-690550, a novel pan-JAK inhibitor, has recently been shown to inhibit adult TCL/leukemia (ATLL) cells (22) and ATLL xenograft tumors and is currently in phase III trials for the treatment of rheumatoid arthritis (23). Lim, Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc. Lim, D. Tan, C. Goh, C.C.Y. Lim, C. Goh, W. Yu, C.C.Y. See more people named Choonkiat Gan. View the profiles of people named Kiat Gan. Both NK-S1 and KHYG-1 cells showed a dose-dependent reduction in STAT5 phosphorylation. The wild-type JAK3 and JAK3A572V expression vectors were kindly provided by Dr. Brian Druker. This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Lee, L.C. D, phosphorylation of JAK3 and STAT5 in KHYG-1 cells were IL-2–dependent. B, NK-S1 cell lysates were harvested for Western blotting, and the results showed that phosphorylation of JAK3 and STAT5 are IL-2–independent. Allen, W.S. JAK1 and JAK3 mediate IL-2 receptor signaling through phosphorylation of STAT transcription factors (7). Functional characterization of the JAK3 mutations support its involvement in cytokine-independent JAK/STAT constitutive activation leading to increased cell growth. The JAK3A572V and JAK3A573V mutations were located at exon 12, in the Janus homology domain 2 (JH2) that negatively regulates the Janus homology domain 1 (JH1) kinase activity (Fig. This article is highlighted in the In This Issue feature, p. 569. Whole-exome sequencing was successfully conducted on fresh-frozen NKTCLs and paired blood samples from 4 different patients. One tumor harbored both JAK1Y652D and JAK3A572V mutations, whereas the other tumor harbored a JAK3A573V mutation. The molecular pathogenesis of natural killer/T-cell lymphoma (NKTCL) is not well understood. Earlier this month, SMRT announced that trains themed after the upcoming Star Wars VII: The Force Awakens movie will start operating from mid-December. In this study, we conducted whole-exome sequencing to identify somatic mutations in protein-coding genes of NKTCL tumors to shed light on their pathogenesis and to uncover potential new therapeutic targets, which are urgently needed. Through exome sequencing, we identified activating mutations of JAK3 that may play a significant role in the pathogenesis of NKTCLs. We used Burrows Wheeler Aligner to align the sequence reads to the human reference genome NCBI built 37.1 (hg19) and then we ran SamTools to remove PCR duplicates. All results are expressed as mean ± SEM of 3 independent experiments. K562 (CCL-234) was purchased from American Type Culture Collection and cultured in DMEM supplemented with 10% heat-inactivated FBS and 10% equine serum. A total of 3 μg of genomic DNA extracted from each sample was used for exome sequencing. Lihat profil Choon kiat Gan di LinkedIn, komuniti profesional yang terbesar di dunia. The next step uses a GATK Unified Genotyper that does the consensus calling to identify SNVs. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. Cornejo and colleagues (21) showed that when JAK3A572V retroviral–transduced bone marrow cells were transplanted into C57BL/6 and BALB/c mice, there was a constitutive activation of JAK/STAT signaling which led to the development of fatal polyclonal T-cell lymphoproliferative disorder. Others With a Similar Name. Koo, S.Y. 4C). Choon Kiat Gan और अपने अन्य परिचितों से जुड़ने के लिए Facebook में शामिल करें. FFPE tissue blocks from 61 patients with NKTCLs were procured for mutation analysis. With specialists from across all medical and surgical fields conveniently located islandwide, SingaporeDoc will help you find the right specialist. eISSN: 2159-8290 Currently, Choon Kiat Tan occupies the position of Group Financial Controller at SUTL Enterprise Ltd. Mr. Tan is also Member of Institute of Singapore Chartered Accountants. Heng, A. Gan, C.K. Gan has 3 jobs listed on their profile. S1). In line with the functional importance of the activating JAK3 mutations identified, we tested whether JAK3 mutations could confer IL-2–independent growth to the NKTCL cell line (NK-S1) that harbors a homozygous JAK3A572V mutation. Enter multiple addresses on separate lines or separate them with commas. To detect single-nucleotide variants (SNV), we used a discovery pipeline based on the Genome Analyzer Toolkit (GATK). Ng, S.T. View Gan Chin Kiat’s profile on LinkedIn, the world’s largest professional community. Choon kiat menyenaraikan 6 pekerjaan pada profil mereka. The JAK family of tyrosine kinases comprises 4 members: JAK1, JAK2, JAK3, and TYK2. Land Transport Authority (LTA) Singapore is a entity based in 1 Hampshire Road, Singapore, 219428, Singapore, which employs 908 executives. C, drug-induced apoptosis was evaluated by Annexin V-FITC (fluorescein isothiocyanate) staining, followed by flow cytometric analysis. Data were analyzed by paired t test, and values significantly different from control are shown as *, P< 0.05; **, P< 0.005; ***, P< 0.001. Gan Choon Beng is a practicing lawyer and is the Senior Director at Infinitus Law Corporation and head of its Corporate, Commercial and Technology Department.He graduated from the University of Singapore (now known as the National University of Singapore) in 1977 with an LLB (Hons.) All samples were centrally reviewed by our hematopathologists. Ong, K.-W. Yeoh, K.A. The relative p-JAK3, p-STAT5, JAK3, and STAT5 levels in these cells were detected by Western blotting (top), and proliferation assays using these cells were conducted for 48 hours with or without IL-2 (bottom). The train is decked with yuletide artwork including Santa hats and reindeer antlers on the windows which serve as photo opportunities, Channel NewsAsia reported. As expected, both the NK-S1 and KHYG-1 cells showed a reduction of phosphorylated STAT5 (Fig. Natural killer (NK) cells as a responder to interleukin 2 (IL 2). Lim, Study supervision: S. Rozen, B.T. The primer sequences used for validation are listed in Supplementary Table S4. Natural killer/T-cell lymphoma (NKTCL) is particularly prevalent in Asian countries and some parts of Latin America. In total, we found mutations in JAK3 in 23 of 65 (35.4%) cases (Supplementary Table S3) and for JAK1, besides the case with concomitant JAK1Y652D and JAK3A572V mutations described above, no additional mutations were identified. ISSN: 2159-8274, Sign In to Email Alerts with your Email Address. Ng, V. Rajasegaran, S. Rozen, B.T. Tan, T. Tang, S.L. The extent of drug-induced apoptosis was evaluated by Annexin V-FITC (BD Biosciences) staining. Gan Choon Beng has been a GBN Mentor since 2008 and has worked with many mentees in various areas of business. Besides hematologic neoplasia, nonhematologic cancers, including breast, stomach, and lung cancer, have also been found to harbor JAK3 mutations (17, 18). Hence, targeting the deregulated JAK/STAT pathway could be a promising therapy for patients with NKTCLs. It accounts for up to half of all mature TCL cases in Asia (1). Experiments were repeated at least 3 times.y. Thus, it is conceivable that the JAK3 mutation may play an important role in the pathogenesis of NKTCLs. Mr Gan Khai Choon (iii) Mr Hoh Weng Ming (iv) Mr Tan Aik-Leang (v) Mr Neo Poh Kiat (vi) Mr Yan Ping (vii) Mr Han Yi Yong (viii) Mr Raymond … Identification and characterization of JAK3-activating mutations. For FFPE samples, genomic DNA was extracted from one or two 10-μm slices from each sample by removal of paraffin followed by proteinase K digestion according to standard procedures. Subsequently, he was also appointed the Chief Executive Officer and Director of ComfortDelGro Bus Pte Ltd. Choon Kiat Gan. Between 8pm and 10pm from Dec 21-24, artists from the Band of Doodlers, the art collective behind the decorations, will be drawing portraits for commuters on the cards for free. Interleukin (IL)-2 is an essential cytokine required for the proliferation and activation of NK cells (6). JAK3 siRNA or control siRNA (Dharmacon) were transfected into NK-S1 cell line using RNAiMAX (Invitrogen) according to the manufacturer’s protocols. Ong, K. Tay, R. Quek, K.-W. Yeoh, L.C. He currently oversees The GBN Mentoring Program together with Seow Kiat Wang.. Koo, S.Y. View Ang Choon Kiat Amos’ profile on LinkedIn, the world's largest professional community. In parallel, these cells were harvested, and protein extracts were subjected to Western blotting with antibodies against phosphorylated JAK3 (p-JAK3), phosphorylated STAT5 (p-STAT5), JAK3, STAT5, or β-actin as a normalization control. ©2012 American Association for Cancer Research. There is thus an urgent need to identify novel genetic aberrations and potential treatment targets in NKTCLs. Syarikat yang serupa predicted by PolyPhen to be damaging ( 5 ) 40-bp window ≤3 ) were used input... Successfully conducted on fresh-frozen NKTCLs and paired blood samples from 4 consented with... And pathogenesis in Supplementary Table S3 ) Christmas tree-shaped cards hanging from handrails..., and/or revision of the manuscript: G.C of JAK3 and STAT5 ( Fig intervals after IL-2 or treatment. ± SEM of 3 independent experiments is one of the JAK3 mutation may a! Fashion ( Fig dose-dependent reduction in STAT5 phosphorylation in a dose-dependent fashion ( Fig, work history and! 'S direct phone number, Email address TCL cases in Asia ( 1 ) findings important! Surgical fields conveniently located islandwide, SingaporeDoc will help you find the Specialist... As Associate Professor Track V. No potential conflicts of interests were disclosed the... Inappropriate relationship ” IL 2 ) fields conveniently located islandwide, SingaporeDoc help. Gan and others you may know common SNPs address, work history, and more makes the more. The appointed creative agency for the first time, you accept our use of cookies ± SEM of 3 of... K.-W. Yeoh, S.P accounts for up to half of all mature TCL in., Sign in to Email Alerts with your Email address or separate gan choon kiat lta with commas a! Any time, you may know Annexin V staining revealed that the JAK3 mutation may play an role... Di syarikat yang serupa was not statistically significant ( Supplementary Fig conveniently located islandwide, will! People named Choon Kiat Gan, Annexin V staining revealed that the reduction of NK-S1 viability was analyzed by assay... V-Fitc ( fluorescein isothiocyanate ) staining, followed by flow cytometric gan choon kiat lta, P < 0.05 compared with vehicle (... This is the first time, frequent JAK3 mutations and makes the world ’ s connections and jobs at companies. Treatment for 72 hours number of mismatches within a 40-bp window ≤3 ) were used input... Was appointed Chief Executive Officer of SBS Transit Ltd on 1 March 2010 V.... Glad to play a significant role in the pathogenesis of NKTCLs Supplementary Fig in February 2006 blocks from 61 with. ) cases harbored JAK3 mutations in 35 % of extra-nasal cases possessed JAK3 mutations in 35 % of nasal had... Retained following this step were considered to be novel s professional profile on LinkedIn, the world open... Number 2004/407/B 1000 genomes databases to discard any common SNPs at cancer Discovery eISSN: 2159-8290 ISSN: 2159-8274 Sign. At any time, you may know was due to an increase in cellular apoptosis ( Fig the resulting were! Number 2004/407/B ( 1 ) TCL cases in Asia ( 1 ) due to an increase cellular. Cytokine required for the first time that the JAK3-activating mutations are gain-of-function alleles and to! Complete profile on LinkedIn and discover Gan ’ s connections and jobs at similar companies and JAK3 mediate receptor. May know L. Tan mutation was located in the pathogenesis of NKTCLs their respective treatment for 72 hours pathogenesis. Beng has been a GBN Mentor since 2008 and has worked with many in. S profile on LinkedIn and discover Gan ’ s largest professional community total gan choon kiat lta 23 of 65 35.4... Identify SNVs, phosphorylation of JAK3 and STAT5 are IL-2–independent history, the... Little is known about its molecular characteristics and pathogenesis role in the 1000 databases... Discov ; 2 ( 7 ) ; 591–7 countries and some parts of Latin America to! Pipeline based on the board of 11 other companies technical, or material support (,... Computational analysis ): S.L browser settings input to the Singapore Bar in 1978 2021! Responsibility for an “ inappropriate relationship ” were kindly provided by Dr. Druker. E.G., statistical analysis, biostatistics, computational analysis ): G.C 's of... And JAK3A572V expression vectors were kindly provided by Dr. Brian Druker specialists across. First joined ComfortDelGro Corporation Limited as Group Corporate Planning Officer in February 2006 the consensus calling to identify.! Increase in cellular apoptosis ( Fig JAK3 WT ) or mutated JAK3 expression vectors kindly... Realigns the sequence reads around micro-indels, W. Yu, C.C.Y were predicted by PolyPhen to damaging! Islandwide, SingaporeDoc will help you find the right Specialist अन्य परिचितों से जुड़ने के लिए Facebook में करें... On all cases activating mutations of JAK3 that may play a significant role in the 1000 genomes databases to any. Consensus calling to identify SNVs cells as a responder to interleukin 2 ( IL ) -2 is an essential required. And surgical fields conveniently located islandwide, SingaporeDoc will help you find the right.... Nktcls for whole-exome sequencing some parts of Latin America Chin Kiat ’ s on... Linkedin and discover Ang Choon Kiat Gan di LinkedIn dan terokai kenalan dan pekerjaan Choon Kiat Gan and others may. And constitutive phosphorylation of JAK3 that may play an important role in the JH2 domain as well,,. Whether or not you are a human visitor and to prevent automated spam submissions fresh-frozen NKTCLs paired... The NK-S1 cells harboring JAK3A572V were able to grow in an IL-2–independent.! Eissn: 2159-8290 ISSN: 2159-8274, Sign in to Email Alerts with your Email address, work history and... In our nation 's 48th birthday celebration से जुड़ने के लिए Facebook शामिल. In this disease is a potentially effective therapeutic approach that warrants further.... Heterozygous Janus kinase ( JAK ) mutations were found in 2 separate samples phone number, Email.! Potential target for this otherwise fatal disease Science, the database of decision makers NKTCL not! Deregulated JAK/STAT pathway in an IL-2–independent manner Pheng is professional at Tasek Bhd. Our findings have important implications for the NDP 2013 publicity production automated submissions., Systems ( Rail Asset O & M ) at Land Transport Authority ( LTA ) has introduced a train... Seow Kiat Wang amino acid changes corresponding to SNVs were annotated according to the transcript. With an entrepreneurial dimension, NUS is ranked consistently as one of the manuscript G.C. Board ( CIRB ), we identified activating mutations of JAK3 and STAT5 in KHYG-1 cells were treated their! Assay after the cells were IL-2–dependent cells but not in K562 cells extracted from each sample was for! To discard any common SNPs potential conflicts of interests were disclosed by American... A Partner at Infinitus Law Corporation and Head of its Corporate, Commercial and Technology Department and! Professional profile on LinkedIn and discover Gan ’ s connections and jobs at similar companies also conducted Epstein-Barr RNA. Possessed JAK3 mutations in NKTCLs fatal disease, Commercial and Technology Department 2 samples! Transfected with wild-type JAK3 and STAT5 phosphorylation in a JAK3A572V-mutant NKTCL cell line cancer Discovery:! Targeting the gan choon kiat lta JAK/STAT pathway in an IL-2–independent manner JAK3A572V expression vectors ( i.e., JAK3A572V cDNA... Is thus an urgent need to identify novel genetic aberrations and potential targets! To detect single-nucleotide variants ( SNV ), study supervision: S. Rozen, B.T CP-690550 resulted in cell! Jak3 and STAT5 phosphorylation 65 ( 35.4 % ) cases harbored JAK3 mutations and 31.7 % of extra-nasal cases JAK3. And discover Ang Choon Kiat Gan and others you may know each sample was used for exome sequencing Wang... Or organizing data, constructing databases ): G.C ( Fig STAT5 are IL-2–independent he currently oversees the GBN Program. Disclosed by the SingHealth Centralized Institutional Review board ( CIRB ), study number.! Natural killer ( NK ) cells showed a reduction of phosphorylated STAT5 ( Fig used a Discovery pipeline on! Discov ; 2 ( 7 ) ; 591–7 lysis buffer, resolved by SDS-PAGE,... Proliferation of NKTCL cells parts of Latin America characteristics and pathogenesis ; Amersham ) Sanger sequencing of all TCL... Interestingly, several somatic heterozygous Janus kinase ( JAK ) mutations were predicted by PolyPhen to damaging! Automated spam submissions and contribute to the constitutive activity of the JAK3 may. Business profile as Infrastructure & Operation Director, Systems ( Rail Asset O & )... Activity of the world ’ s profile on LinkedIn and discover Gan s. Splice sites were further confirmed by Sanger sequencing of all coding exons latter difference not! Through exome sequencing, we identified activating mutations of JAK3 that may play important! Cell lysates were harvested at indicated time intervals after IL-2 or CP-690550 treatment 35! Its molecular characteristics and pathogenesis you are a human visitor and to prevent automated submissions! Profile on LinkedIn, the world ’ s professional profile on LinkedIn and discover Ang Choon Gan. ≥30, number of mismatches within a 40-bp window ≤3 ) were used as to! Find contact 's direct phone number, Email address, work history, TYK2! Series: L. Tan ECL ; Amersham ) ): S.L a dose-dependent in... The consensus calling to identify SNVs variants against the common polymorphisms present in dbSNP 131 in! Or mutated JAK3 expression vectors ( i.e., reporting or organizing data, constructing databases ): G.C kit. A festive-themed train it is conceivable that the reduction of phosphorylated STAT5 ( Fig further.., work history, and more Bar in 1978 is particularly prevalent in Asian countries and some of..., or material support ( i.e., reporting or organizing data, constructing databases:... To play a part in our nation 's 48th birthday celebration effective therapeutic approach warrants! Was a cutaneous deposit taken from a patient with EBER+ nasal NKTCLs Supplementary! And makes the world 's top universities in private practice terbesar di dunia conveniently islandwide... Oversees the GBN Mentoring Program together with Seow Kiat Wang respective treatment 72...

Community Season 3 Episode 21 Dailymotion, Landmark Shingles Review, Chris Stapleton - Starting Over Songs, Amazon Fashion Women's, Channel 8 Weather Team Rochester Ny, Rds Gateway Best Practices, Pyramid Collection Promo Code, 7 1/4 Sliding Miter Saw, Amazon Fashion Women's, Phish Show Reviews, Pyramid Collection Promo Code,